Start a Bio-One Franchise in 2025

Franchise 500 Rank: N/R Not ranked last year

Initial investment: $135K - $221K

Units as of 2025: 137 9.6% over 3 years

As one of the globe’s only crime scene clean-up franchises, Bio-One, which was founded in 2008, offers premiere decontamination and biohazard cleaning services at reasonable costs, restoring homes, buildings, and other spaces to livable conditions following an incident. Since beginning to franchise in 2011, the company has expanded with over 100 franchised locations all across the U.S.

If you’re seeking a franchise opportunity that will make a difference in people’s lives, Bio-One could be the brand for you. As a franchisee, you have the chance to change customers' lives for the better.

Why You May Want to Start a Bio-One Franchise

There are far too many violent crimes committed in America each year. While we are incapable of controlling these numbers, there is a way to help the victims and their families move on with their lives. Nobody dreams of a crime scene market, but as a present-day reality, you can choose to respond productively for the people in your community.

Entering this market with no concrete track record is very difficult. But with Bio-One’s ten-plus years of experience, you can leverage the company’s long-standing national contracts, insurance partners, TPA networks, and proven marketing strategies. You may be poised to become the next big thing in your business circuit with your brand new Bio-One franchise.

What Might Make a Bio-One Franchise a Good Choice?

Multiple times in the past few years, Bio-One has ranked in BIZ Experiences’s Franchise 500 based on an evaluation of more than 150 data points in the areas of costs and fees, size and growth, franchisee support, brand strength, and financial strength and stability.

A key benefit of choosing the brand is that you can usually get your franchise up and running as soon as you finish training. Other physical store-based franchise models typically require much more money and time—sometimes up to a year before you can set up shop.

To be part of the Bio-One team, you should make sure you’re financially ready for an initial investment that will include a franchise fee and other startup costs. You should also be prepared for ongoing fees, which will include advertising, royalty, and renewal fees. Franchisees will also need to meet the company's set net worth and liquid capital requirements.

How Do You Open a Bio-One Franchise?

Biological waste cleanup is a complicated term, but franchising with Bio-One may be simple. To learn more about the franchise opportunity, submit an inquiry form. Bio-One may reach out to you and provide the information you need about the business, as well as an overview of your role as a franchisee. All these details will come in a Franchise Disclosure Document, and you must take time to go through it as closely as possible to avoid surprises later on.

Once you have read the Franchise Disclosure Document, Bio-One has reviewed your information, and you both decide to commit to a partnership, you may sign the franchise agreement and pay the initial fees. After that, the company will put you through biohazardous waste disposal training before you are allowed to begin your business. The franchise team will be with you as you prepare for the challenging responsibilities that come with your new Bio-One franchise.

Company Overview

About Bio-One

Industry	Maintenance
Related Categories	Biohazard Cleaning, Miscellaneous Maintenance Businesses, Miscellaneous Services, Cleaning/Restoration
Founded	2008
Parent Company	Five Star Franchising
Leadership	Danessa Itaya, President
Corporate Address	761 W. Spring Creek Pl., #200 Springville, UT 84663
Social	Facebook, Twitter, LinkedIn, Instagram, YouTube

Business Overview

Franchising Since	2011 (14 years)
# of employees at HQ	11
Where seeking	This company is offering new franchises throughout the US.
# of Units	137 (as of 2025)

Information for Franchisees

Here's what you need to know if you're interested in opening a Bio-One franchise.

Financial Requirements & Ongoing Fees

Here's what you can expect to spend to start the business and what ongoing fees the franchisor charges throughout the life of the business.

Initial Franchise Fee Definition: The initial fee paid to a franchisor to join their system What you need to know: Found in Item 5 of the FDD, this may be a flat fee, or may vary based on territory size, experience, or other factors.The franchise fee is an up-front (one-time) cost that a new franchisee pays to the franchisor. This fee is usually due at the signing of the franchise agreement and covers the right to use the franchisor's trademarks, name, and related business systems.	$60,000
Initial Investment Definition: The total amount necessary to begin operation of the franchise What you need to know: The initial investment includes the franchise fee, along with other startup expenses such as real estate, equipment, supplies, business licenses, and working capital. This is outlined in a chart in Item 7 of the FDD, showing a range of possible costs from low to high.	$134,645 - $221,095
Net Worth Requirement Definition: The minimum net worth you must have in order to qualify to become a franchisee of this company What you need to know: Net worth is the value of a person's assets minus liabilities. Assets include cash, stocks, retirement accounts, and real estate. Liabilities include items like mortgages, car payments, and credit card debt.	$250,000
Cash Requirement Definition: The minimum liquid capital you must have available in order to qualify to become a franchisee of this company.	$60,000
Veteran Incentives Definition: A discount or other incentive offered to military veterans who buy a franchise with this company.	$9,000 off franchise fee
Royalty Fee Definition: A ongoing fee paid to the franchisor on a regular basis. What you need to know: Most franchisors require franchisees to pay an ongoing royalty fee, which is detailed in Item 6 of the FDD. This fee is typically a percentage of weekly or monthly gross sales, but may also be a flat weekly, monthly, or annual fee.	7.5%
Ad Royalty Fee Definition: An going fee paid to the franchisor on a regular basis to support advertising or marketing efforts. What you need to know: This may also be called advertising fee, marketing fee, brand fund fee, and more, but the basic purpose is the same-- to support promotion of the brand systemwide. As with the royalty fee, it is detailed in Item 6 of the FDD, and can be a percentage of weekly or monthly gross sales or a weekly, monthly, or annual fee.	2%
Term of Agreement Definition: The length of time your franchise agreement will last. What you need to know: Franchise terms are typically anywhere from 5 to 20 years in length, but are sometimes instead dependent on factors such as the term of your lease. Once your term is up, you may have the option to renew your agreement, typically for a smaller fee than the original franchise fee.	10 years
Is franchise term renewable?	Yes

Financing Options

Some franchisors offer in-house financing, while others have relationships with third-party financing sources to which they refer qualified franchisees.

Third Party Financing	Bio-One has relationships with third-party sources which offer financing to cover the following: franchise fee, startup costs, equipment, inventory, accounts receivable, payroll

Training & Support Offered

Franchisors offer initial training programs and a variety of ongoing support options to help franchisees run their businesses.

On-The-Job Training	10 hours
Classroom Training	30 hours
Ongoing Support	Purchasing Co-ops Newsletter Meetings & Conventions Grand Opening Online Support Security & Safety Procedures Field Operations Proprietary Software Franchisee Intranet Platform
Marketing Support	Ad Templates National Media Regional Advertising Social Media SEO Website Development Email Marketing

Operations

Additional details about running this franchise.

Is absentee ownership allowed?	No
Can this franchise be run from home/mobile unit? Definition: The business can be run from your home and/or a vehicle, and it is not necessary to have a retail facility, office space, or warehouse.	Yes
Can this franchise be run part time? Definition: This business can be run by the owner on a part-time basis (less than 40 hours per week) and/or as a side business; it is not necessary for the business to be open/run full-time.	Yes
# of employees required to run	2
Are exclusive territories available? Definition: An exclusive territory is a fixed area in which you are given the right to operate and in which no other units of the same franchise may be opened. What you need to know: Territory size may be based on factors such as radius, population size, zip codes, and more. Details can be found in Item 12 of the FDD.	No